ATP10D and male infertility: Therefore, the genotypes of the mice used in this study were 10A+/+10D−/− (WT) and 10A−/−10D−/− (KO) and it was formally possible that the male infertility observed in the 10A−/−10D−/− (KO) mice was caused by loss of both Atp10A and Atp10D. To determine if Atp10A deficiency was sufficient to cause this phenotype, we generated 10D+/+ mice via CRISPR-mediated repair of the nonsense mutation back to the wild-type glutamine codon and confirmed the genotype of the mice via sequencing (Supplementary Figure S4A).