External datasets validation confirmed significant downregulation of GRM8 and ESR1 in GERD patients (Figures 7B, C), and in IPF patients, GRM8 was significantly downregulated (p = 0.0005), and ESR1 was significantly upregulated (p = 0.0397) (Figures 7E, F). This evidence concerns the gene ESR1 and gastroesophageal reflux disease.