BCR and chronic myelogenous leukemia, BCR-ABL1 positive: According to the results shown inFigure 5A,C, after infection with the Cas9-GFP/dual-BA-sgRNA lentivirus, the BCR-ABL fusion genomic sequence in the primary CML cells was almost completely disrupted; after PCR amplification, the longer DNA fragment (wild-type) disappeared, and only the mutated fragments appeared.