Colonization of tumorigenic ETBF to the intestinal epithelium can trigger multiple signaling pathways, including E‐cadherin cleavage,[49] Wnt/β‐catenin pathway,[50] pStat3 signaling,[51] and NF‐κB signaling,[52] which have been linked to pre‐oncogenic events for colorectal cancer (CRC).[36, 53] While these events have not been induced by live ETBF in in vitro gut models, we investigated whether the CRC signaling events reported in ETBF‐colonized in vivo models and BFT‐treated cells could be replicated in our μGut model. This evidence concerns the gene CDH1 and colorectal carcinoma.