Through an analysis of the enrichment levels of distinct metabolic state macrophages across varying polarization statuses, ISUP histological grades, and tumor grades (Fig. 4D), we unveiled that GLY+ Macrophages primarily exhibited enrichment within M2-polarized macrophages, while AA+ Macrophages, FATTY+ Macrophages, and PURINE+ Macrophages emerged as notably enriched within M1-polarized macrophages. This evidence concerns the gene TEAD1 and neoplasm.