We did not examine the effects of vitamin D on the gene expression of TGF-β, but consumption of vitamin D2 with doses of 60,000, 80,000, 100,000 IU/weekly for vitamin D insufficient, deficient, severely insufficient in patients with chronic hepatitis C for 6 weeks has no significant effect on serum TGF-β1, TIMP-1, MMP-9 and P3NP compared to placebo group [45]. This evidence concerns the gene TIMP1 and chronic hepatitis C virus infection.