To rule out an impact of AF1q silencing on MYCN gene expression, qRT-PCR was used to quantify MYCN mRNA levels in Kelly and Lan-5 cells after infection with AF1q shRNA and control virus (Supplementary Fig. 7A), at a time point when N-Myc protein levels were clearly reduced as shown by western blotting (Supplementary Fig. 7B). The gene discussed is MYCN; the disease is infection.