To explore the involvement of CBX7 and IGF-1R in FOXC1-mediated stemness regulation in ESCC, we conducted transfection experiments using plasmids overexpressing CBX7 and IGF-1R in KYSE-150-shControl-LV, KYSE-150-shFOXC1-LV, ECA-109-shControl-LV, and ECA-109-shFOXC1-LV cell lines. Here, IGF1R is linked to esophageal squamous cell carcinoma.