We validated that IGF-1 enhanced the proliferative ability of tumor cells (Fig. 5E), elevated the protein and mRNA expression of cancer stem cell markers CD133 and CD44 (Fig. 5F, G), along with enhanced tolerance to cisplatinoid drugs (Fig. 6H, I) and in vitro sphere formation ability(Fig. 5J). The gene discussed is PROM1; the disease is cancer.