Surprisingly, only S100A9 positive cells are specifically and significantly observed in necrotic areas (Figure 4E), and thus we observed a significantly large infiltration of CD11B+S100A9+MCHII- immunosuppressive myeloid cells (MDSCs) in tumors with high necrosis compared to low necrosis male GBM tumors (Figure 4F and G). The gene discussed is S100A9; the disease is glioblastoma.