In our analyses of structural and functional changes of blood vessels in the tumor, we found that male GBM patients with high-ESR1 expression are associated with an increase in αSMA pericyte coverage on tumor vessels (40.68-fold, Figure 2D), endothelial tight junctional molecule VE-cadherin (12.02-fold, Figure 2E) and distribution of collagen type IV + basement membrane coverage (1.68-fold, Figure 2F) on tumor vessels. This evidence concerns the gene ACTA1 and glioblastoma.