To shed more mechanistic light on the impact of Nsp1 on NFAT activation, cells were transfected with NFAT-Luc and TK-Luc 24 hours prior to infection with WT SARS-CoV-2 and mutant ΔNsp1 viruses at an M.O.I. of 0.01. The gene discussed is SH2D3A; the disease is infection.