To assess whether IL-7 treatment can increase the rate of HIV infection in resting primary human CD4+ T cells, we cultured the cells overnight in complete RPMI media with very low levels of IL-2 (10 IU/mL) and IL-7 (5 ng/mL), and then, the cells were infected with a replication-competent HIV-1 virus strain (HIV-LAI.2 WT, Subtype B, CXCR4 tropic) by spinoculation. Here, CXCR4 is linked to HIV infectious disease.