Therefore, the protective effects of order Bifidobacteriales, family Bifidobacteriaceae, and genus Bifidobacterium on NB may primarily manifest in two aspects: first, Bifidobacterium and its metabolic byproducts can inhibit the progression of NB by suppressing the PI3K/Akt/mTOR signaling pathway; second, Bifidobacterium influences the immune response within the tumor microenvironment by promoting the expression of IL-27, thereby preventing the development of NB. Here, IL27 is linked to neuroblastoma.