Collectively, our study suggests that mmu-miR-10a-5p, mmu-miR-488-3p, and mmu-miR-1224-5p may modulate anxiety-like behavior through AMPK, cAMP, PI3K-Akt signaling pathways involving Creb1 following fecal transplantation from mice with anxiety disorders. Here, CREB1 is linked to anxiety disorder.