In both 6DT1 and 4T1 cell lines, KD of NANOS1, PUM2, and CPSF4 did not consistently affect primary tumor growth but did significantly reduce the number of metastatic nodules on the lungs compared to shScramble control (shScr) (Fig. 1c–n and Fig. S1c–n). The gene discussed is NANOS1; the disease is neoplasm.