For this purpose, we performed Annexin V apoptosis detection assay (Fig. 3M,N,and O).The results showed that after interfering with EGR1 expression in CFPAC-1 and PANC-1 cells, apoptotic cells were significantly increased after knockdown of EGR1 compared with sh-NC.The EdU assay showed a decrease in the proliferative capacity of pancreatic cancer cells after silencing EGR1 expression(Fig. 3P). This evidence concerns the gene ANXA5 and pancreatic neoplasm.