EGR1 and familial pancreatic carcinoma: The results of both qRT-PCR and Western blot analysis showed that the expression of EGR1 was increased in different pancreatic cancer cell lines compared to normal pancreatic ductal epithelial cells.We detected the content of EGR1 in pancreatic cancer cells after gemcitabine intervention by qRT-PCR and found that gemcitabine intervention induced upregulation of EGR1 and the rise in expression increased with increasing dose and time (Fig. 1E,F,G,and H).