Notably, alkaline phosphatase (ALP) levels significantly decreased in the treated group compared to the untreated PBC mouse models (119.10 ± 6.20 vs. 82.36 ± 12.50 U/L, P < 0.05), while alanine aminotransferase (ALT) and aspartate aminotransferase (AST) levels remained unaltered (Fig. 3C). This evidence concerns the gene GPT and primary biliary cholangitis.