Moreover, to find out the proper expression level of exogenous TRIM72 that matches the endogenous expression level of TRIM72 during viral infection, a dose-dependent TRIM72 expression experiment was carried out by transfecting different concentrations of TRIM72 expressing vectors (0.25 μg, 0.5 μg, 0.75 μg, and 1.0 μg) into N2a cell, and the expression levels of TRIM72 was determined to compare with the expression level of TRIM72 during RABV infection. Here, TRIM72 is linked to viral infectious disease.