As the scRNA-seq data do not support discernment of whether immune cells have infiltrated tumors or are merely present in the sampled materials, we performed multispectral immunofluorescence (mIF) staining on CD4+ T cells, CD8+ T cells, M1 macrophages, and M2 macrophages in formalin-fixed paraffin-embedded (FFPE) tumor tissues of P1-P5. This evidence concerns the gene CD4 and neoplasm.