RGS4 and neoplasm: A functional enrichment analysis based on gene ontology (GO) annotation was performed to further characterize the biological function of tumor clusters: the tumor cluster A DEGs showed functional enrichment for terms including generation of metabolites and energy, response to oxygen levels, response to hypoxia, and ATP metabolic process and some notably upregulated genes of tumor cluster A included known energy metabolism-related genes such as NDUFA4L2, COX4I2, RGS4, and AQP1 (Figure 4C).