We also noticed the early expression of PNMT, while neuron-specific genes such as MAP2, MAP1B, L1CAM, and CNTN1 were up-regulated later in the pseudotime trajectory (Figure 5F), suggesting the decrease of epinephrine synthesis and the appearance of neuron-like phenotypes during tumor evolution, consistent with previously reported neuron-like phenotypes in PCC cells with high expression of RET (Califano et al., 1995; Powers et al., 2003; Powers et al., 2009). The gene discussed is L1CAM; the disease is neoplasm.