There were no significant differences in TTE on univariate and multivariate analysis when patients were stratified according to systemic therapy, presence of brain metastasis symptoms, number of brain metastases, PD-L1 score, whether they received upfront local therapy or presence and number of other sites of metastatic disease (Table 2 and Supplementary Table 4). The gene discussed is CD274; the disease is metastatic neoplasm.