TLR4 and autoimmune polyendocrinopathy: To affirm whether the TLR4/NF-κB signaling pathway was involved in the therapeutic mechanism of APS-treated PTZ-kindling mice, we evaluated the protein expressions of TLR4, NF-κB p65, and p-IκBα present in the Hip tissues via western blot analysis (Figure 10), corroborating the enriched results obtained from our previous network pharmacology study.