PTBP3 and cancer: Firstly, we detected PTBP3 expression levels in different RCC cancer cell lines, data showed PTBP3 was higher expressed in 786-O, ACHN and Ketr3 RCC cell lines than normal kidney derived HK2 cell (Figure S1) To investigate the role of PTBP3 in RCC progression, 786-O and ACHN cells were stably infected with lentivirus-mediated PTBP3 shRNA and control shRNA or transfected with PTBP3 overexpression and control lentivirus (Figures 2A and 2B).