Moreover, analysis of RNAseq data of primary endothelial cells from Ch25h null mice treated with vehicle or reserpine and then treated with EVs from the colon cancer cell line MC38, which also release EVs with NUPR1 [Supplementary Figure 3A], demonstrated that cells treated with NUPR1-rich EVs upregulated NUPR1 expression while reserpine suppressed NUPR1 expression [Figure 8E]. Here, CH25H is linked to malignant colon neoplasm.