Based on the phase 1b study conducted in TB-free adults, the immunity induced by H56:IC31 and H4:IC31 vaccines and repeated with the BCG vaccine, in terms of efficacy and safety, it was observed that the H56:IC31 and H4:IC31 vaccines induced CD4+ T cells identifying vaccine-matched antigens and H56 and H4 specific IgG binding antibodies. The gene discussed is CD4; the disease is tuberculosis.