Subsequent ROC curve analysis of 20 lymphocyte subsets and the cytokines IL-6 and IFN-γ based on tumor response revealed significant differences in CD3+ T-cell counts, CD4+ T-cell counts, CD8+ T-cell counts, CD8+CD28+ T-cell frequency, CD8+CD38+ T-cell frequency, CD8+PD-1+/CD4+PD-1+ T-cell ratio, and the concentration of IL-6 in predicting tumor response (all p < 0.05) (Supplementary Table 3). This evidence concerns the gene CD28 and neoplasm.