After the testification of knockout efficiency (Fig. S2A), we also conducted several cell phenotype experiments and found that the cell cycle of H1299 was greatly arrested (Fig. S2F), the proliferation ability was inhibited (Fig. S2G), the migration and invasion abilities were blocked significantly (Fig. S2, H and I) after NSUN2 knockout, which was further provided the evidence for NSUN2 tumor-promoting role in NSCLC. This evidence concerns the gene NSUN2 and neoplasm.