In subjects with SUD/MDD, we observed significantly increased aggrecan (Acan) (Fig. 3C: p < 0.03), as well as chondroitin synthase 1 (Chsy1) mRNA (Figs. 3D, S3B: p < 0.0001; with significant effects of duration of AUD and tissue pH). This evidence concerns the gene CHSY1 and major depressive disorder.