CD28 and non-small cell lung carcinoma: Human tumors from 16 patients with treatment naïve NSCLC were harvested, mechanically and enzymatically digested, and treated with isotype control Ab, toripalimab or pembrolizumab in the presence of anti-CD3 and anti-CD28 Abs, for 6 and 24 h (hr) (Fig. 6A).