First, blood-based biosamples were measured at a single point in time, while serum levels of NfL and GFAP are known to gradually increase hours after symptom onset and reaching maximum plasma levels at different timepoints days after stroke onset.7,29 Biomarker trajectories created from repeated measurements may contain additional information regarding the development of the short- and long-term functional outcome. This evidence concerns the gene GFAP and Stroke.