CD40LG and cancer: Then, once imDCs are obtained, they are ex vivo matured by the addition of a cocktail of several cytokines, such as TNF-α, prostaglandin-E1 (PGE1), prostaglandin-E2 (PGE2) and/or picibanil (OK-432) or CD40 ligand (CD40L) [54,55], and loaded with antigens through several pathways: whole cell antigens or tumor lysates, synthetic antigenic peptides, tumor DNA or mRNA, exosomes derived from cancer cells [54,56], Figure 2.