Figure S5 also revealed that immune response-associated cell numbers of B cells, T cells and various cytokines all markedly improved after immunization. Among them, IFN-γ, an indicator of the Th1-type immune response, is an important assessment for the development of porcine streptococcal vaccines [23,57], and it was strongly induced in rMVSS-simulated immunization. In addition IL-2, which is associated with the prevention of pathogenic bacterial infections [58], was also strongly stimulated to be upregulated with multiple immunizations against MVSS. The gene discussed is IFNG; the disease is bacterial infectious disease.