The negative impact on BoHV-1 replication associated with the loss of COG6 expression was further evidenced by delayed production of the VP26-GFP protein after high or low MOI infection of COG6-/- KO cells with BoHV-1 VP26-GFP virus compared to the Cas9+/+ control cells (Figure 4c). Here, COG6 is linked to infection.