New urgent requirements for fast, sensitive, accurate, and inexpensive tools for the early diagnosis of PC devalue the traditional PSA detection methods [16], such as ELISA, radioimmunoassay immunoradiometric assay, and time-resolved immunofluorescence assay, which have a complex operation, are difficult to miniaturize, and can have a limited sensitivity [23,24,25,26]. The gene discussed is KLK3; the disease is pachyonychia congenita.