To determine whether the P1 and P2 probes can detect BA.1 VOC gRNA and sgRNA, Vero cells expressing high levels of transmembrane serine protease 2 (TMPRSS2) were infected with 0.5 m.o.i. of the SARS-CoV-2 BA.1 strain at 3, 6, and 12 h post infection and probed using the P1 and P2 probes (Figure 8B, panels 2, 4, and 6). The gene discussed is TMPRSS2; the disease is infection.