Since macrophages are classified into inflammatory macrophages (M1) and anti-inflammatory macrophages (M2), we isolated hepatic macrophages from the livers of CCl4-induced chronic hepatitis mice and analyzed the populations of positive cells for CD80 (M1 marker) or CD206 (M2 marker) by flow cytometry (Figure 5B) according to the previous report [36]. The gene discussed is CD80; the disease is chronic hepatitis.