RNA sequencing data from the Cancer Cell Line Encyclopedia (CCLE) database revealed low transcript abundance of ALK in CCA cell lines (log2TPM + 1 < 0.5) (Figure 1G) [27] and analysis of “The Cancer Genome Atlas” (TCGA) dataset revealed no significant difference in ALK mRNA expression between normal bile duct (tumor adjacent normal) (N = 9) and CCA tumor (T = 36), and both exhibited notably low expressions (log2 (FKPM) < 0.5) (Figure 1H). This evidence concerns the gene ALK and cancer.