The transfection efficiency and cytotoxicity of TBP2-GFP plasmid polyplex NPs were evaluated in various cell lines including tool cells (293T), a cervical cancer cell line (HeLa, Me180 and SiHa), and a nasopharyngeal carcinoma cell line (C666-1 and SCC-7). Here, TBPL2 is linked to nasopharyngeal carcinoma.