Significantly altered serum low-density lipoprotein cholesterol (LDL-C), total cholesterol, total bile acid (TBA), and triglyceride levels. Significantly differed glycoprotein, pH, and free Ca2+ of bile. Altered deoxycholic acid, chenodeoxycholic acid, and cholic acid levels, exhibiting the reverse development of bile composition in patients with cholecystolithiasis taking probiotics, thereby diminishing gallstones. This evidence concerns the gene ART4 and cholecystolithiasis.