Statistically significant reduction (30%, p < 0.05) in symptoms, including decreased anxiety and depression and improved sexual function (p < 0.05); no differences were seen in serum concentrations of E2, FSH, LH, and SHBG. Post hoc analyses revealed a significant increase in E2 would only be detected at a 30% increase. This evidence concerns the gene PLOD1 and depressive symptom measurement.