In addition, in IgE immunoblotting using serum from patients with WDEIA, a 55-kDa band corresponding to ω5-gliadin was not observed at 5–10% CIS for either BW (lanes 3, 4; Figure 2b) or 1BS-18M (lanes 7, 8; Figure 2b); however, a faint band was observed at the area of 40 kDa for 1BS-18M with 5% CIS (lane 7; Figure 2b). This evidence concerns the gene IGHE and in situ carcinoma.