We performed an in vitro competition binding assay, PET imaging, and ex vivo biodistribution studies in preclinical PSMA-expressing LNCaP and FAP-expressing HEK293T:hFAP tumor models to evaluate the potential of [68Ga]Ga-AV01084 and [68Ga]Ga-AV01088 for prostate cancer imaging. Here, FAP is linked to Familial prostate cancer.