These non-genetically engineered T-cells, targeting two of the most common transcription molecules overexpressed in leukemias, presented, after 4 stimulations with overlapping peptides spanning the whole WT1 and PRAME antigens, a memory phenotype with high specificity and cytotoxicity against both leukemia-associated antigens (LAA) in vitro. This evidence concerns the gene WT1 and leukemia.