SMAD2 and breast cancer: Conversely, the conditioned media derived from lungs and scaffolds of MCF7-tumor-bearing mice induced the rapid transcription factor activity of EMT (HIF1, RUNX1, RUNX2, NOTCH1, and SNAIL), pro-metastatic (SMAD1, SMAD2, and HNF1a), and breast cancer maintenance (SP1, and OCT4)-related factors, which were initiated minutes after CM incubation and were sustained until the 4 h timepoint (Figure 8b).