We found on both the whole HSR (Figure 4d, left panel) and HSR sections (Figure 4d, right panel) that Tspan8’s ectopic expression was sufficient to decrease melanoma cell stiffness, whereas Tspan8’s inhibition increased it, confirming that Tspan8 expression could enhance melanoma dermal invasion by reducing melanoma cell stiffness. Here, TSPAN8 is linked to melanoma.