Thus, in a murine PDAC model, the shRNA-mediated reduction of Nestin expression led to decreased tumor volume and hepatic metastases [37,38], which is in line with our results demonstrating that Nestin-expressing Panc1 Holoclone cells showed a higher self-renewal capacity and invasive properties in vitro, and formed a higher number of tumors in vivo compared to Panc1 Paraclone cells. This evidence concerns the gene NES and neoplasm.