Moreover, a loss in the mean para-vascular aquaporin-4 (AQP4, Figure 3C) immunoreactivity was detected with the progression of DM (1M, 26.46; 3M, 16.50; non-DM, 29.92), but only showed a trend of statistical significance (p = 0.09) for the 3M-DM group compared with the non-DM group. This evidence concerns the gene AQP4 and diabetes mellitus.