In this study, we noticed that the results of transcriptome sequencing after knocking out SRPK1 indicated a significant decrease in the activity of the Wnt/β-catenin and JAK-2/STAT-3 signaling pathways, suggesting that SRPK1 may directly regulate Wnt/β-catenin and JAK-2/STAT-3 to promote the occurrence of gliomas. Here, SRPK1 is linked to glioma.