A decreased percentage of PD-1+ T1-like CD4+CD8+ T cells in OB (57% ± 24.9 vs. nOB: 68% ± 26.2, p > 0.05; Supplementary Table S9) was also observed, especially in Class III obesity (50% ± 24.3). Here, CD4 is linked to obesity due to melanocortin 4 receptor deficiency.