In order to identify the best reference genes for gene expression studies in peripheral blood and/or bone marrow samples of acute leukemia patients, a qPCR assay based on TaqMan detection for the expression analysis of the six selected genes (ACTB, ABL, GAPDH, HPRT, TBP and RPLP0) was used. The gene discussed is RPLP0; the disease is acute leukemia.