To evaluate IEC responses specifically during TNBS colitis and treatment with I3C, CECs were enriched from experimental groups (Vehicle, I3C, TNBS + Veh, and TNBS + I3C), and enrichment purity was assessed by flow cytometry for EPCAM-positive selection, which was found to be between 72 and 80% (Figure 1C). The gene discussed is EPCAM; the disease is colitis.