Zhou et al. demonstrated that BM cells from patients with Philadelphia chromosome-negative myeloproliferative neoplasms (MPNs) showed a significantly increased expression in NLRP3 inflammasome-related genes (NLRP3, NF-κB1, CARD8, IL-1β, and IL-18) and the increased expression was associated with the JAK2V617F mutation, white blood cell counts and splenomegaly. This evidence concerns the gene CARD8 and Splenomegaly.